|
KMID : 1159320120140040431
|
|
Korean Journal of Vision Science
2012 Volume.14 No. 4 p.431 ~ p.424
|
|
|
Synergistic Antimicorbial Effect of Epigallocatechin-gallate (EGCG) and Polyhexamethylene-biguanide (PHMB) Mixture
|
|
Lim Sang-Mook
Lee Koon-Ja
|
|
|
Abstract
|
|
|
|
Purpose: Antimicrobial activities of Epigallocatechin-gallate (EGCG) and Polyhexamethylenebiguanide (PHMB)/EGCG mixture were investigated against microbes associated with infective keratitis and its possibility as the preservative in the contact lens care solution. Methods: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, Candida albicans and Fusarium solai which are ISO required strains, were inoculated in each liquid media and then activated for 24 hours at the optimal temperature of each strain. Activated strains were incubated for 12 hours using 96-well plate on media containing 0.0000125~0.0004% of PHMB and 0.0025~0.3% of EGCG. The PHMB/EGCG mixture contained minimum inhibitory concentration (MIC, 0.00005%) of PHMB and 0.0125~0.1% of EGCG. Optical density of the culture media were measured at 600 nm for the antimicrobial activity. Result: The MIC of EGCG against ISO required infectious microbes were higher (100-1,500X) than that of PHMB that antibacterial activity of EGCG was low. MIC of PHMB/EGCG mixture against ISO required strains were 0.00005%/0.05% which is 25% of that of PHMB that PHMB/EGCG mixture showed higher microbial activity. Conclusion: PHMB/EGCG mixture showed synergistic antimicrobial activity against ISO required infectious microbes that could lower PHMB concentration in the multipurpose solution. The results get in this study will be cornerstone in the development of contact lens care solutions.
|
|
|
KEYWORD
|
|
|
Polyhexamethylene-biguanide (PHMB), Epigallocatechin-gallate (EGCG), Minimum inhibitory concentration (MIC), Half maximal inhibitory concentration, Antimicrobial sy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|